MTT (CAS 298-93-1) — formula C18H16N5S.Br, molecular weight 414.3.
| CAS Number | 298-93-1 |
|---|---|
| Molecular Formula | C18H16N5S.Br |
| Molecular Weight | 414.3 |
| Melting Point | 195 °C (decomposes) |
| Applications | Uses: Used in biological assays, [HSBD], Tetrazolium salts are used for the detection of dehydrogenase activity or any other enzyme systems where redox equivalents are generated. Due to this feature, they are an extremely useful tool in academic and clinical research as well as for many diagnostic applications, eg: (1) aids and cancer research, cell and molecular biology: for testing cell proliferation and cell viability and for cytotoxicity tests (2) Enzyme diagnostics and clinical chemistry: for the assay of dehydrogenase activity and other enzyme systems which form redox equivalents, for the determination of dehydrogenase substrate concentrations, for the detection of glucose, ethanol, glycerol and other substrates which develop redox equivalents in a coupled reaction (3) Immuno histochemistry: for the detection of alkaline phosphatase (4) Histology and pathology: for the histochemical detection of dehydrogenase (5) In seed nurseries: for testing seed viability., Tetrazolium salts are widely used for detecting redox potential of cells for viability, cytotoxicity and proliferation assays. ... Reduction of MTT remains the most common assay for tetrazolium salt-based viability testing., Tetrazolium salts for detecting redox potential in living cells and tissues: 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. Applications: superoxide generation by fumarate reductase and nitric oxide synthase, mitochondrial dehydrogenase activity, cell viability and proliferation, neuronal cell death, platelet activation, tumor cell adhesion and invasion, multidrug resistance, in vitro toxicity testing, from table, The MTT assay is frequently used in anti-HIV and antineoplastic drug screening for the evaluation of cell survival and proliferation., For more Uses (Complete) data for METHYLTHIAZOLETETRAZOLIUM (6 total), please visit the HSDB record page., General Manufacturing Information: 2H-Tetrazolium, 2-(4, 5-dimethyl-2-thiazolyl)-3, 5-diphenyl-, bromide (1:1): ACTIVE, The MTT assay is simple, accurate and yields reproducible results. This method has been developed originally by Mossman in 1983. The key component is (3-[4, 5-dimethylthiazol-2-yl]- 2, 5-diphenyl tetrazolium bromide) or MTT. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, leading to the formation of purple crystals which are insoluble in aqueous solutions. The crystals are re-dissolved in acidified isopropanol and the resulting purple solution is measured spectrophotometrically. An increase or decrease in cell number results in a concomitant change in the amount of formazan formed, indicating the degree of cytotoxicity caused by the test material (IC50)., A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision., The authors, compared the MTT assay to the standard clonogenic assay and had good agreement of surviving fraction, of murine solid tumor cells, after radiation doses of 2 and 4 Gy. It is possible, therefore, to adapt the MTT assay for use with cell suspensions prepared directly from fresh murine tumors. This may provide a methodology for the determination of the clinical radiosensitivity of tumors including fresh clinical tumor specimens., A microcytotoxicity assay employing a tetrazolium salt has been adapted for testing the response of human leukemic blast cells to a variety of chemotherapeutic agents. After exposure to various concentrations of drugs, the viability of fresh leukemic blast cells was measured using a tetrazolium salt, MTT, which is converted to blue formazan crystals by living cells. The amount of formazan produced was quantitated using a microtiter plate spectrophotometer. |
Documented applications: Uses: Used in biological assays, [HSBD], Tetrazolium salts are used for the detection of dehydrogenase activity or any other enzyme systems where redox equivalents are generated. Due to this feature, they are an extremely useful tool in academic and clinical research as well as for many diagnostic applications, eg: (1) aids and cancer research, cell and molecular biology: for testing cell proliferation and cell viability and for cytotoxicity tests (2) Enzyme diagnostics and clinical chemistry: for the assay of dehydrogenase activity and other enzyme systems which form redox equivalents, for the determination of dehydrogenase substrate concentrations, for the detection of glucose, ethanol, glycerol and other substrates which develop redox equivalents in a coupled reaction (3) Immuno histochemistry: for the detection of alkaline phosphatase (4) Histology and pathology: for the histochemical detection of dehydrogenase (5) In seed nurseries: for testing seed viability., Tetrazolium salts are widely used for detecting redox potential of cells for viability, cytotoxicity and proliferation assays. ... Reduction of MTT remains the most common assay for tetrazolium salt-based viability testing., Tetrazolium salts for detecting redox potential in living cells and tissues: 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. Applications: superoxide generation by fumarate reductase and nitric oxide synthase, mitochondrial dehydrogenase activity, cell viability and proliferation, neuronal cell death, platelet activation, tumor cell adhesion and invasion, multidrug resistance, in vitro toxicity testing, from table, The MTT assay is frequently used in anti-HIV and antineoplastic drug screening for the evaluation of cell survival and proliferation., For more Uses (Complete) data for METHYLTHIAZOLETETRAZOLIUM (6 total), please visit the HSDB record page., General Manufacturing Information: 2H-Tetrazolium, 2-(4, 5-dimethyl-2-thiazolyl)-3, 5-diphenyl-, bromide (1:1): ACTIVE, The MTT assay is simple, accurate and yields reproducible results. This method has been developed originally by Mossman in 1983. The key component is (3-[4, 5-dimethylthiazol-2-yl]- 2, 5-diphenyl tetrazolium bromide) or MTT. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, leading to the formation of purple crystals which are insoluble in aqueous solutions. The crystals are re-dissolved in acidified isopropanol and the resulting purple solution is measured spectrophotometrically. An increase or decrease in cell number results in a concomitant change in the amount of formazan formed, indicating the degree of cytotoxicity caused by the test material (IC50)., A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision., The authors, compared the MTT assay to the standard clonogenic assay and had good agreement of surviving fraction, of murine solid tumor cells, after radiation doses of 2 and 4 Gy. It is possible, therefore, to adapt the MTT assay for use with cell suspensions prepared directly from fresh murine tumors. This may provide a methodology for the determination of the clinical radiosensitivity of tumors including fresh clinical tumor specimens., A microcytotoxicity assay employing a tetrazolium salt has been adapted for testing the response of human leukemic blast cells to a variety of chemotherapeutic agents. After exposure to various concentrations of drugs, the viability of fresh leukemic blast cells was measured using a tetrazolium salt, MTT, which is converted to blue formazan crystals by living cells. The amount of formazan produced was quantitated using a microtiter plate spectrophotometer..
MTT is a chemical compound with CAS registry number 298-93-1 and molecular formula C18H16N5S.Br (molecular weight 414.3).
CAS number: 298-93-1
Its molecular formula C18H16N5S.Br comprises 18 C, 16 H, 5 N, 1 S, 1 Br atoms.
Also known as: MTT · 噻唑蓝
The CAS registry number of MTT is 298-93-1.
Molecular formula C18H16N5S.Br, molecular weight 414.3.
It is also registered under: MTT, 噻唑蓝.
Recorded applications: Uses: Used in biological assays, [HSBD], Tetrazolium salts are used for the detection of dehydrogenase activity or any other enzyme systems where redox equivalents are generated. Due to this feature, they are an extremely useful tool in academic and clinical research as well as for many diagnostic applications, eg: (1) aids and cancer research, cell and molecular biology: for testing cell proliferation and cell viability and for cytotoxicity tests (2) Enzyme diagnostics and clinical chemistry: for the assay of dehydrogenase activity and other enzyme systems which form redox equivalents, for the determination of dehydrogenase substrate concentrations, for the detection of glucose, ethanol, glycerol and other substrates which develop redox equivalents in a coupled reaction (3) Immuno histochemistry: for the detection of alkaline phosphatase (4) Histology and pathology: for the histochemical detection of dehydrogenase (5) In seed nurseries: for testing seed viability., Tetrazolium salts are widely used for detecting redox potential of cells for viability, cytotoxicity and proliferation assays. ... Reduction of MTT remains the most common assay for tetrazolium salt-based viability testing., Tetrazolium salts for detecting redox potential in living cells and tissues: 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. Applications: superoxide generation by fumarate reductase and nitric oxide synthase, mitochondrial dehydrogenase activity, cell viability and proliferation, neuronal cell death, platelet activation, tumor cell adhesion and invasion, multidrug resistance, in vitro toxicity testing, from table, The MTT assay is frequently used in anti-HIV and antineoplastic drug screening for the evaluation of cell survival and proliferation., For more Uses (Complete) data for METHYLTHIAZOLETETRAZOLIUM (6 total), please visit the HSDB record page., General Manufacturing Information: 2H-Tetrazolium, 2-(4, 5-dimethyl-2-thiazolyl)-3, 5-diphenyl-, bromide (1:1): ACTIVE, The MTT assay is simple, accurate and yields reproducible results. This method has been developed originally by Mossman in 1983. The key component is (3-[4, 5-dimethylthiazol-2-yl]- 2, 5-diphenyl tetrazolium bromide) or MTT. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, leading to the formation of purple crystals which are insoluble in aqueous solutions. The crystals are re-dissolved in acidified isopropanol and the resulting purple solution is measured spectrophotometrically. An increase or decrease in cell number results in a concomitant change in the amount of formazan formed, indicating the degree of cytotoxicity caused by the test material (IC50)., A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision., The authors, compared the MTT assay to the standard clonogenic assay and had good agreement of surviving fraction, of murine solid tumor cells, after radiation doses of 2 and 4 Gy. It is possible, therefore, to adapt the MTT assay for use with cell suspensions prepared directly from fresh murine tumors. This may provide a methodology for the determination of the clinical radiosensitivity of tumors including fresh clinical tumor specimens., A microcytotoxicity assay employing a tetrazolium salt has been adapted for testing the response of human leukemic blast cells to a variety of chemotherapeutic agents. After exposure to various concentrations of drugs, the viability of fresh leukemic blast cells was measured using a tetrazolium salt, MTT, which is converted to blue formazan crystals by living cells. The amount of formazan produced was quantitated using a microtiter plate spectrophotometer..
Independent reference data for MTT (CAS 298-93-1), compiled from public chemistry databases.
| CAS 号 | 298-93-1 |
|---|---|
| PubChem CID | 64965 |
| 分子式 | C18H16N5S.Br |
| IUPAC 名称 | 2-(3,5-diphenyltetrazol-2-ium-2-yl)-4,5-dimethyl-1,3-thiazole bromide |
| UNII（FDA） | EUY85H477I |
| Molecular Weight | 414.3 |
|---|---|
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 3 |
| Exact Mass | 413.03098 |
| Monoisotopic Mass | 413.03098 |
| Topological Polar Surface Area | 75.7 |
| Heavy Atom Count | 25 |
| Melting Point | 195 °C (decomposes) |
|---|
Thiazolyl blue; 298-93-1; MMT Tetrazolium; Thiazolyl Blue Monotetrazolium; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide; 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide; EUY85H477I; 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide; NSC-60102; NSC-367079; CHEBI:53233; 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide; 2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; RefChem:897686
| GHS 通报统计 | 2% (1 / 51) |
|---|
| GHS 通报统计 | 2% (1 / 51) |
|---|---|
| 已通报的危害声明 | H315 (92.2%), H319 (92.2%), H335 (90.2%), H341 (90.2%) |
噻唑蓝 · CAS 298-93-1 · C18H16N5S.Br. UNII EUY85H477I. Melting Point: 195 °C (decomposes).
Source: PubChem / Wikidata; the batch COA governs where values differ.
Supplier: Jinan Shenghe Chemical Co., Ltd.
中文页面: 噻唑蓝(MTT)